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KMID : 0613820180280091022
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Journal of Life Science
2018 Volume.28 No. 9 p.1022 ~ p.1029
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Anti-inflammatory and Cellular Proliferation Effects of Ethanol Extracts from 5 Kinds of Oriental Medical Plants
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Jung Min-Hwa
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Abstract
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This study was carried out to search for the anti-inflammatory activities of ethanol extracts obtained from 5 kinds of oriental medical plant; Pleuropterus multiflorus extract (PME), Acorus calamus L. extract (ACE), Lithospermum erythrorhizon Siebold & Zucc. extract (LEE), Xanthium strumarium L. extract (XSE), Lonicera japonica extract (LJE), which have traditionally been used as a drug in oriental medical plants in Korea. XSE showed cytotoxicity at 100, 200 μg/ml concentration in RAW264.7 cells (p<0.05) and ACE showed cytotoxicity at 200 μg/ml concentration in RAW264.7 cells (p<0.05). But other oriental medical plants did not showed cytotoxicity was observed in RAW264.7 cells below 200 μg/ml concentration. These extracts at non-toxic concentrations showed anti-inflammatory effects. PME, ACE, XSE and LJE showed a concentration-dependent inhibitory effect on NO production and PGE2 production in LPS-induced RAW264.7 cells. In particular, XSE showed the highest NO production inhibition (52.9 μg/ml, IC50) as well as the highest PGE2 production inhibition at 50 μg/ml (73.6%). ACE and LEE showed cell proliferation effects on HaCaT keratinocyte cells. Especially, LEE showed 21.1, 53.5 and 99.6% proliferative activity by incubation for 1, 3, 5days at 100 μg/ml concentration. ACE also showed 11.2, 26.0% proliferative activity for 1day and 3days at 10 μg/ml concentration. As a result of this study, ethanol extracts obtained from 5 kinds of oriental medical plant showed anti-inflammatory activity and HaCaT cell regeneration effect.
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KEYWORD
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Anti-inflammatory, cell proliferation, cytotoxicity, ethanol extracts, oriental medical plants
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